Technical Data

036406
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_002097
Gene ID
3015
Gene #
H2AFZ
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-H2AFZ, CT (H2AFZ, H2AZ, Histone H2A.Z)
H2AFZ, H2AZ, Histone H2A.Z

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
H2AFZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the C-terminal region of human H2AFZ.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Marques, M., et al. Epigenetics 5(4):267-272(2010)|Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)|Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)|Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009) :|Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)
USBio References
No references available
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