036406
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_002097Gene ID
3015Gene #
H2AFZShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-H2AFZ, CT (H2AFZ, H2AZ, Histone H2A.Z)
H2AFZ, H2AZ, Histone H2A.Z
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq].
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
H2AFZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the C-terminal region of human H2AFZ.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Marques, M., et al. Epigenetics 5(4):267-272(2010)|Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)|Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)|Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009) :|Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)USBio References
No references available