036423
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
O96004; NP_004812.1Gene ID
9421Gene #
HAND1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-HAND1, ID (HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1)
HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HAND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human HAND1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 18(19):3567-3578(2009) Martinez Hoyos, J., et al. Oncogene 28(6):876-885(2009) Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 17(10):1397-1405(2008) Morin, S., et al. J. Biol. Chem. 280(37):32272-32278(2005) Hill, A.A., et al. Mol. Cell. Biol. 24(22):9835-9847(2004)USBio References
No references available