Technical Data

036443
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001018238.1; NP_006109.2; O00165
Gene ID
10456
Gene #
HAX1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HAX1, CT (HAX1, HS1BP1, HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1)
HAX1, HS1BP1, HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from the C-terminal region of human HAX1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Johns, H.L., et al. J. Gen. Virol. 91 (PT 11), 2677-2686 (2010) :|Mekkawy, A.H., et al. Biochem. Biophys. Res. Commun. 399(4):738-743(2010)|Han, J., et al. J. Biol. Chem. 285(29):22461-22472(2010)|Germeshausen, M., et al. Haematologica 95(7):1207-1210(2010)|Burnicka-Turek, O., et al. BMC Cell Biol. 11, 28 (2010) :
USBio References
No references available
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