Rabbit Anti-HBB, CT (HBB, Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, LVV-hemorphin-7) (FITC)
HBB, Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, LVV-hemorphin-7
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry (Not Tested) and FLISA. Othre applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 80-107 in the C-terminal region of human HBB, UniProt Accession #P68871. Cellular Localization: Red blood cells.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HBB. Species Sequence Homology: bovine, chicken, equine, mouse, porcine, rat, rabbit, sheep
