Technical Data

036446
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_005321.1; P02100
Gene ID
3046
Gene #
HBE1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HBE1v (HBE1, HBE, Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain)
HBE1, HBE, Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3'

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 55-83 in the central region of human HBE1v, UniProt Accession #NP_005321.1; P02100.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HBE1v.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Nuinoon, M., et al. Hum. Genet. (2009) In press 2. Tachavanich, K., et al. Southeast Asian J. Trop. Med. Public Health 40(2):306-316(2009) 3. Naka, I., et al. Biochem. Genet. 46 (11-12), 708-711 (2008) 4. Winichagoon, P., et al. Transl Res 152(4):178-184(2008) 5. Das, B., et al. Ann. Hum. Biol. 35(4):422-431(2008)
USBio References
No references available
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