Technical Data

036574
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_003600.2
Gene ID
8479
Gene #
HIRIP3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HIRIP3, ID (HIRIP3, HIRA-interacting protein 3)
HIRIP3, HIRA-interacting protein 3

The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Recently, several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the HIRIP3 gene product binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HIRIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-316 amino acids from the Central region of human HIRIP3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Assrir, N., et al. Biol. Chem. 388(4):391-398(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Olsen, J.V., et al. Cell 127(3):635-648(2006)
USBio References
No references available
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