Technical Data

036605
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_066403.2
Gene ID
126961; 333932; 653604
Gene #
HIST2H3A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HIST2H3A, CT (HIST2H3A, Histone H3.2, Histone H3/m, Histone H3/o)
HIST2H3A, Histone H3.2, Histone H3/m, Histone H3/o

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HIST2H3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the C-terminal region of human HIST2H3A.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Neumann, H., et al. Mol. Cell 36(1):153-163(2009)|Hurd, P.J., et al. J. Biol. Chem. 284(24):16575-16583(2009)|Yuan, J., et al. Cell Cycle 8(11):1747-1753(2009)|Chang, Q., et al. J. Hepatol. 50(2):323-333(2009)|Kobza, K., et al. BMB Rep 41(4):310-315(2008)
USBio References
No references available
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