036608
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_066406.1Gene ID
3018Gene #
HIST1H2BBShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Histone H2B, NT (HIST1H2BB, H2BFF, HIST1H2BE, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f)
HIST1H2BB, H2BFF, HIST1H2BE, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HIST1H2BB/HIST1H2BE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BB/HIST1H2BE.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)|Pavri, R., et al. Cell 125(4):703-717(2006)|Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)|Zhu, B., et al. Mol. Cell 20(4):601-611(2005)|Golebiowski, F., et al. Mol. Cell. Biochem. 279 (1-2), 133-139 (2005) :USBio References
No references available