Technical Data

036612
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
3020
Gene #
H3F3A; H3F3B
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Histone H3 (K9) (H3F3A, H3.3A, H3F3, Histone H3.3)
H3F3A, H3.3A, H3F3, Histone H3.3

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Teng, H., et al. J. Biol. Chem. 284(39):26368-26376(2009)|Garcia, B.A., et al. J. Biol. Chem. 282(10):7641-7655(2007)|Morris, S.A., et al. J. Biol. Chem. 282(10):7632-7640(2007)|Loyola, A., et al. Mol. Cell 24(2):309-316(2006)|Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
USBio References
No references available
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