Technical Data

036613
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_060880.3
Gene ID
55355
Gene #
HJURP
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HJURP, CT (HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9)
HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9

HJURP is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 559-588 in the C-terminal region of human HJURP.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HJURP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sebastiani, P., et al. Science (2010). 2. Shuaib, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1349-1354(2010). 3. Hu, Z., et al. Breast Cancer Res. 12 (2), R18 (2010). 4. Dunleavy, E.M., et al. Cell 137(3):485-497(2009). 5. Foltz, D.R., et al. Cell 137(3):472-484(2009)
USBio References
No references available
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