Technical Data

036618
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_002109.1
Gene ID
3109
Gene #
HLA-DMB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HLA-DMB, ID (HLA-DMB, DMB, RING7, HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein)
HLA-DMB, DMB, RING7, HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HLA-DMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-133 amino acids from the Central region of human HLA-DMB.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rinderknecht, C.H., et al. Immunology 131(1):18-32(2010)|Bailey, S.D., et al. Diabetes Care (2010) In press :|Davila, S., et al. Genes Immun. 11(3):232-238(2010)|Ferrante, A., et al. J. Immunol. 184(3):1153-1158(2010)|Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
USBio References
No references available
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