Technical Data

036642
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000182.2; NP_001159531.1
Gene ID
3155
Gene #
HMGCL
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HMGCL, ID (HMGCL, Hydroxymethylglutaryl-CoA lyase, mitochondrial, 3-hydroxy-3-methylglutarate-CoA lyase)
HMGCL, Hydroxymethylglutaryl-CoA lyase, mitochondrial, 3-hydroxy-3-methylglutarate-CoA lyase

HMGCL belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HMGCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from the Central region of human HMGCL.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Fu, Z., et al. J. Biol. Chem. 285(34):26341-26349(2010)|Pierron, S., et al. Arch Pediatr 17(1):10-13(2010)|Menao, S., et al. Hum. Mutat. 30 (3), E520-E529 (2009) :|Lin, W.D., et al. Clin. Chim. Acta 401 (1-2), 33-36 (2009) :|Carrasco, P., et al. Mol. Genet. Metab. 91(2):120-127(2007)
USBio References
No references available
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