Technical Data

036656
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IF WB
Crossreactivity
Hu
Gene ID
3172
Gene #
HNF4A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HNF4A, NT (HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4)
HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4

HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.

Applications
Suitable for use in Western Blot, Immunofluorescence, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116~145 amino acids from the N-terminal region of human HNF4A.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human HNF4A

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010) |Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009)|Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009) |Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006) |Hong, Y.H., et al. J. Biol. Chem. 278(30):27495-27501(2003)
USBio References
No references available
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