Rabbit Anti-HP, ID (HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain)
HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000 Immunohistochemistry (Praffin): 1:500. Antigen retrieval using EDTA pH 9.0 is recommended. Indirect Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 296-322 in the central region of human HP.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HP. Species Crossreactivity: mouse.
