Technical Data

036863
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu Rt
Accession #
NP_851998.1; NP_002156.2
Gene ID
3397
Gene #
ID1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ID1, ID (ID1, BHLHB24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1)
ID1, BHLHB24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry and Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:100. Heat induced antigen retrieval with EDTA Buffer, pH 9.0 is recommended. Flow Cytometry (Indirect): 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 66-93 iof human ID1. Species Sequence Homology: mouse.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ID1. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Qian, T., et al. Oncogene 29(43):5818-5827(2010). 2. Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010). 3. Yang, J., et al. Circ. Res. 107(2):252-262(2010). 4. Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010). 5. Geng, H., et al. Cancer Res. 70(8):3239-3248(2010)
USBio References
No references available
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