Technical Data

036864
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_002158.3
Gene ID
3399
Gene #
ID3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ID3, CT (ID3, 1R21, BHLHB25, HEIR1, DNA-binding protein inhibitor ID-3, Class B basic helix-loop-helix protein 25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3)
ID3, 1R21, BHLHB25, HEIR1, DNA-binding protein inhibitor ID-3, Class B basic helix-loop-helix protein 25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ID3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the C-terminal region of human ID3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Shuno, Y., et al. J. Surg. Res. 161(1):76-82(2010)|Doran, A.C., et al. Circ. Res. 106(7):1303-1311(2010)|McGeachie, M., et al. Circulation 120(24):2448-2454(2009)|Higgins, S., et al. Endocrinology 150(9):4044-4055(2009)|Trabosh, V.A., et al. Exp. Dermatol. 18(4):387-395(2009)
USBio References
No references available
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