Technical Data

036866-HRP
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Gene ID
3400
Gene #
ID4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-ID4, NT (ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4) (Azide free) (HRP)
ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4

ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed, paraffin embedded tissues Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human ID4. Species Sequence Homology: porcine.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ID4. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009). 2. Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009)
USBio References
No references available
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