Technical Data

037128
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_003592.2
Gene ID
8467
Gene #
SMARCA5
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog)
SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human ISWI.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010)|He, X., et al. Biochemistry 47(27):7025-7033(2008)|Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
USBio References
No references available
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