Technical Data

037171-FITC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NP_872381.2
Gene ID
284359
Gene #
IZUMO1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-IZUM1, NT (IZUMO1, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo) (FITC)
IZUMO1, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).

Applications
Suitable for use in Western Blot and FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 77-105 from the N-terminal region of human IZUM1
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human IZUM1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ellerman, D.A., et al. Mol. Reprod. Dev. 76(12):1188-1199(2009). 2. Inoue, N., et al. Biochem. Biophys. Res. Commun. 377(3):910-914(2008). 3. Granados-Gonzalez, V., et al. Fertil. Steril. 90(4):1246-1248(2008). 4. Yatsenko, A.N., et al. Hum. Mol. Genet. 15(23):3411-3419(2006). 5. Inoue, N., et al. Nature 434(7030):234-238(2005).
USBio References
No references available
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