Rabbit Anti-JHDM1D, ID (JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D)
JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
JHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-325 amino acids from the Central region of human JHDM1D.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
