Technical Data

037206
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Gene ID
216850
Gene #
Kdm6b
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-JMJD3, ID (Kdm6b, Jmjd3, Kiaa0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3)
Kdm6b, Jmjd3, Kiaa0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 964~993 amino acids from the center region of mouse JMJD3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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