Technical Data

037711
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_034823.1; NP_001155905.1
Gene ID
16818
Gene #
Lck
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Lck, NT (Lck, Lsk-t, Proto-oncogene tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK)
Lck, Lsk-t, Proto-oncogene tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK

Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR (By similarity).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Lck antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of Mouse Lck.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Visan, I., et al. J. Immunol. 185(8):4609-4617(2010)|Chrobak, P., et al. J. Immunol. 185(7):3948-3959(2010)|McCoy, M.E., et al. J. Immunol. 185(6):3285-3294(2010)|Veillette, A., et al. J. Immunol. 185(5):2650-2657(2010)|Hashimoto-Tane, A., et al. Mol. Cell. Biol. 30(14):3421-3429(2010)
USBio References
No references available
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