038063-PE
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
PEIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IHC WBCrossreactivity
HuAccession #
P43357Gene ID
4102Gene #
MAGEA3Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
Rabbit Anti-MAGEA3, CT (MAGEA3, MAGE3, Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, MAGE-3 antigen) (PE)
MAGEA3, MAGE3, Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, MAGE-3 antigen
MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
Applications
Suitable for use in FLISA, Western Blot, Immunohistochemistry and Flow Cytometry (Not Tested). Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 283-314 in the C-terminal region of human MAGEA3.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A/G affinity chromatography
Specificity
Recognizes human MAGEA3 at ~35kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Guo, J., et al., Exp. Mol. Pathol. 74(2):140-147 (2003). Consogno, G., et al., Blood 101(3):1038-1044 (2003). Rogner, U.C., et al., Genomics 29(3):725-731 (1995). Gaugler, B., et al., J. Exp. Med. 179(3):921-930 (1994). van der Bruggen, P., et al., Science 254(5038):1643-1647 (1991).USBio References
No references available