038097
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Hu MoAccession #
Q6UB28; NP_954697.1Gene ID
254042Gene #
MAP1DShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MAP1D, NT (METAP1D, MAP1D, Methionine aminopeptidase 1D, mitochondrial, Methionyl aminopeptidase type 1D, mitochondrial)
METAP1D, MAP1D, Methionine aminopeptidase 1D, mitochondrial, Methionyl aminopeptidase type 1D, mitochondrial
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).
Applications
Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 306-334 in the N-terminal region of human MAP1D, UniProt Accession #Q6UB28; NP_954697.1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human MAP1D. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 4. Leszczyniecka, M., et al. Oncogene 25(24):3471-3478(2006). 5. Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003)USBio References
No references available