Technical Data

038197
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_073737.1
Gene ID
64844
Gene #
40975
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MARCH7, NT (MARCH7, AXOT, RNF177, E3 ubiquitin-protein ligase MARCH7, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, RING finger protein 177)
MARCH7, AXOT, RNF177, E3 ubiquitin-protein ligase MARCH7, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, RING finger protein 177

MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MARCH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human MARCH7.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Szigyarto, C.A., et al. J. Histochem. Cytochem. 58(4):301-308(2010)|Nathan, J.A., et al. Traffic 9(7):1130-1145(2008)|Muthukumarana, P.A., et al. Int. Immunopharmacol. 6 (13-14), 1993-2001 (2006) :|Flierman, D., et al. Proc. Natl. Acad. Sci. U.S.A. 103(31):11589-11594(2006)|Lim, J., et al. Cell 125(4):801-814(2006)
USBio References
No references available
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