038233
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Hu MoGene ID
8932Gene #
MBD2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MBD2, NT (MBD2, Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2)
MBD2, Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MBD2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Zhu, Y., et al., Cancer 100(9):1853-1858 (2004).|Ghoshal, K., et al., J. Biol. Chem. 279(8):6783-6793 (2004).|Fujita, H., et al., Mol. Cell. Biol. 23(8):2645-2657 (2003).|Patra, S.K., et al., Biochem. Biophys. Res. Commun. 302(4):759-766 (2003).|Lembo, F., et al., Mol. Cell. Biol. 23(5):1656-1665 (2003).USBio References
No references available