038242
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_003916Gene ID
8930Gene #
MBD4Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MBD4, ID (Methyl-CpG-binding Domain 4)
Methyl-CpG-binding Domain 4
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and the MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MBD4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Evertson, S., et al., Anticancer Res. 23(4):3569-3574 (2003).|Yamada, T., et al., Cancer Lett. 181(1):115-120 (2002).|Schlegel, J., et al., Oncol. Rep. 9(2):393-395 (2002).|Petronzelli, F., et al., J. Biol. Chem. 275(42):32422-32429 (2000).|Petronzelli, F., et al., J. Cell. Physiol. 185(3):473-480 (2000).USBio References
No references available