Technical Data

038246
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_066368.2
Gene ID
4154
Gene #
MBNL1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MBNL1, CT (MBNL1, EXP, KIAA0428, MBNL, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein)
MBNL1, EXP, KIAA0428, MBNL, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 255-284 in the C-terminal region of human MBNL1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human MBNL1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sen, S., et al. J. Biol. Chem. 285(33):25426-25437(2010). 2. Arambula, J.F., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16068-16073(2009). 3. Cardani, R., et al. Neuromuscul. Disord. 19(5):335-343(2009). 4. Holt, I., et al. Am. J. Pathol. 174(1):216-227(2009). 5. Teplova, M., et al. Nat. Struct. Mol. Biol. 15(12):1343-1351(2008)
USBio References
No references available
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