038277
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuGene ID
4171Gene #
MCM2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MCM2, CT (MCM2, BM28, CCNL1, CDCL1, KIAA0030, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28)
MCM2, BM28, CCNL1, CDCL1, KIAA0030, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Saade, E., et al. Proteomics 9(21):4934-4943(2009)|Xu, X., et al. EMBO J. 28(19):3005-3014(2009)|Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)|Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)|Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Matsuoka, S., et al. Science 316(5828):1160-1166(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)USBio References
No references available