Technical Data

038410
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001108089.1; NP_001108090.1
Gene ID
4245
Gene #
MGAT1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MGAT1, ID (MGAT1, GGNT1, GLCT1, GLYT1, MGAT, Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I)
MGAT1, GGNT1, GLCT1, GLYT1, MGAT, Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MGAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the Central region of human MGAT1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Henquet, M., et al. Transgenic Res. 19(4):535-547(2010)|Johansson, A., et al. Obesity (Silver Spring) 18(4):803-808(2010)|Bousman, C.A., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (8), 1025-1049 (2009) :|Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :|Saribas, A.S., et al. Biochem. Biophys. Res. Commun. 362(2):381-386(2007)
USBio References
No references available
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