038429
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_036348.2; NP_438112.2Gene ID
11043Gene #
MID2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MID2, CT (MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1)
MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed, paraffin embedded tissues Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 690-717 in the C-terminal region of human MID2. Species Sequence Homology: mouse
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human MID2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lim, J., et al. Cell 125(4):801-814(2006). 2. Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005). 3. Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004). 4. Short, K.M., et al. BMC Cell Biol. 3, 1 (2002). 5. Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)USBio References
No references available