Technical Data

038474
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E
Crossreactivity
Hu
Accession #
NP_005924
Gene ID
4297
Gene #
MLL
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MLL1, CT (KMT2A)
KMT2A

Chromosome 11q23 translocations targeting mixed-lineage leukemia 1 (MLL) gene have been correlated with human acute leukemias, including lymphoid, myeloid, monoblastic and myelomonocytic varieties. More than 3 dozen genes that fuse to with MLL have been identified in various types of malignancies, including AF4, AF9, ENL, CBL, LARG, LPP, GMPS, and FBP17. The 431 kDa MLL protein exhibits greatest abundancy in cerebral cortex, kidney, thyroid, and lymphoid tissues. Inside the cell, MLL is part of a stable multiprotein supercomplex, the majority of which are components of transcription complexes, including TFIID. The MLL multiprotein complex seems to remodel, acetylate, deacetylate, and methylate nucleosomes and/or free histones. MLL contains a SET domain characteristic for histone H3 lysine-4-specific methyltransferases. Oncogenic MLL fusion proteins appear to alter the methyltransferase activity of wild-type MLL.

Applications
Suitable for use in ELISA
Recommended Dilution
ELISA: 1:1,000
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MLL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3873~3902 amino acids from the C-terminal region of human MLL1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Bertrand, F.E., et al., Leukemia 17(12):2454-2459 (2003). Dyson, M.J., et al., Cancer Genet. Cytogenet. 147(1):81-83 (2003). Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100(14):8342-8347 (2003). Ferrando, A.A., et al., Blood 102(1):262-268 (2003). Echlin-Bell, D.R., et al., Hum. Genet. 113(1):80-91 (2003).
USBio References
No references available
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