038593
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_006419.2Gene ID
10573Gene #
MRPL28Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-MRPL28, NT (MRPL28, MAAT1, 39S ribosomal protein L28, mitochondrial, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes)
MRPL28, MAAT1, 39S ribosomal protein L28, mitochondrial, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MRPL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human MRPL28.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Lamesch, P., et al. Genomics 89(3):307-315(2007)|Martin, J., et al. Nature 432(7020):988-994(2004)|Zhang, Z., et al. Genomics 81(5):468-480(2003)|Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)|Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :USBio References
No references available