Technical Data

038601
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_758455.1
Gene ID
116541
Gene #
MRPL54
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MRPL54, ID (MRPL54, 39S ribosomal protein L54, mitochondrial)
MRPL54, 39S ribosomal protein L54, mitochondrial

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MRPL54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the Central region of human MRPL54.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Lamesch, P., et al. Genomics 89(3):307-315(2007)|Zhang, Z., et al. Genomics 81(5):468-480(2003)|Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)|Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995) :
USBio References
No references available
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