Rabbit Anti-MUTYH, ID (MUTYH, MYH, A/G-specific adenine DNA glycosylase, MutY homolog)
MUTYH, MYH, A/G-specific adenine DNA glycosylase, MutY homolog
MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches. MYH is involved in oxidative DNA damage repair. It initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. It possesses both adenine and 2-OH-A DNA glycosylase activities.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Indirect Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Indirect Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 341-367 in the central region of human MUTYH.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human MUTYH. Species Crossreactivity: mouse
