Technical Data

038733
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001138416.1; O14950
Gene ID
103910
Gene #
MYL12B
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MYL12B, NT (MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20kD, Myosin regulatory light chain MRLC2, SHUJUN-1)
MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20kD, Myosin regulatory light chain MRLC2, SHUJUN-1

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MYL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYL12B.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)|Umeda, D., et al. Biochem. Biophys. Res. Commun. 333(2):628-635(2005)|Li, T.B., et al. Acta Biochim. Biophys. Sin. (Shanghai) 36(6):412-418(2004)|Webb, R.C. Adv Physiol Educ 27 (1-4), 201-206 (2003) :|Iwasaki, T., et al. Cell Struct. Funct. 26(6):677-683(2001)
USBio References
No references available
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