Technical Data

038821
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_056076.1
Gene ID
23310
Gene #
NCAPD3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NCAPD3, CT (NCAPD3, CAPD3, KIAA0056, Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3)
NCAPD3, CAPD3, KIAA0056, Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).

Applications
Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NCAPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1078 amino acids from the C-terminal region of human NCAPD3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NCAPD3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : 2. Takemoto, A., et al. Nat. Struct. Mol. Biol. 16(12):1302-1308(2009) 3. Lapointe, J., et al. Am. J. Surg. Pathol. 32(2):205-209(2008) 4. Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) 5. Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007) :
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net