Technical Data

038830
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
O43639; NP_001004720.1; NP_003572.2
Gene ID
8440
Gene #
NCK2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NCK2, NT (NCK2, GRB4, Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta)
NCK2, GRB4, Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Guan, S., et al. J. Invest. Dermatol. 129(8):1909-1920(2009)|Liu, J., et al. PLoS ONE 4 (11), E7805 (2009) :|Akiyama, M., et al. Br J Ophthalmol 92(9):1293-1296(2008)|Park, S., et al. J. Biomol. NMR 34(3):203-208(2006)
USBio References
No references available
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