038871
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
O00217; NP_002487.1Gene ID
4728Gene #
NDUFS8Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-NDUFS8, ID (NDUFS8, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, NADH-ubiquinone oxidoreductase 23kD subunit, TYKY subunit)
NDUFS8, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, NADH-ubiquinone oxidoreductase 23kD subunit, TYKY subunit
This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NDUFS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human NDUFS8.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Bourges, I., et al. Biochem. J. 383 (PT 3), 491-499 (2004) :|Procaccio, V., et al. Neurology 62(10):1899-1901(2004)|Ugalde, C., et al. Hum. Mol. Genet. 13(6):659-667(2004)|Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)USBio References
No references available