Technical Data

038880
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Gene ID
79661
Gene #
NEIL1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NEIL1, ID (NEIL1, Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, Nei-like protein 1)
NEIL1, Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, Nei-like protein 1

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137~167 amino acids from the Central region of human NEIL1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human NEIL1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Zhao, X., et al. Biochemistry 49(8):1658-1666(2010)|Forsbring, M., et al. Carcinogenesis 30(7):1147-1154(2009)|Couve, S., et al. J. Biol. Chem. 284(18):11963-11970(2009)|Muftuoglu, M., et al. J. Biol. Chem. 284(14):9270-9279(2009)|Horikawa, Y., et al. Clin. Cancer Res. 14(23):7956-7962(2008)
USBio References
No references available
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