Technical Data

038881
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001129220.1; NP_001129218.1; NP_659480.1
Gene ID
252969
Gene #
NEIL2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NEIL2, NT (NEIL2, Endonuclease 8-like 2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, Nei-like protein 2)
NEIL2, Endonuclease 8-like 2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, Nei-like protein 2

NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NEIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human NEIL2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)|Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :|Grin, I.R., et al. Biochem. Biophys. Res. Commun. 394(1):100-105(2010)|Kinslow, C.J., et al. Genes Chromosomes Cancer 47(11):923-932(2008)|Zhai, X., et al. Clin. Cancer Res. 14(13):4345-4352(2008)
USBio References
No references available
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