038981
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC IF IHC WBCrossreactivity
HuAccession #
NP_004322.1Gene ID
665Gene #
BNIP3LShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-NIX, ID (BNIP3L, BNIP3A, BNIP3H, NIX, BCL2/adenovirus E1B 19kD protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19kD protein-interacting protein 3A, NIP3-like protein X)
BNIP3L, BNIP3A, BNIP3H, NIX, BCL2/adenovirus E1B 19kD protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19kD protein-interacting protein 3A, NIP3-like protein X
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:10-1:50 Indirect Flow Cytometry: 1:10-1:0 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 52-81 in the centralterminal region of human NIX. Species Sequence Homology: bovine and mouse
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NIX
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Novak, I., et al. EMBO Rep. 11(1):45-51(2010). 2. Bellot, G., et al. Mol. Cell. Biol. 29(10):2570-2581(2009). 3. Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). 4. Papandreou, I., et al. Cell Death Differ. 15(10):1572-1581(2008).5. Liu, W., et al. Neoplasia 10(8):897-907(2008)USBio References
No references available