038997-APC
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
APCIsotype
IgGGrade
Affinity PurifiedApplications
FLISA WBCrossreactivity
Hu MoAccession #
NP_003308.1Gene ID
7080Gene #
NKX2-1Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRabbit Anti-NKX2-1, NT (NKX2-1, NKX2A, TITF1, TTF1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1) (APC)
NKX2-1, NKX2A, TITF1, TTF1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Applications
Suitable for use in Western Blot and FLISA. Other potential application, though not tested is Direct Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human NKX2-1. Species sequence homology: rat.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NKX2-1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Kim, J.H., et al. Acta Cytol. 54(3):277-282(2010) Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010) Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010) Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010) : Cantara, S., et al. Thyroid Res 3 (1), 4 (2010) :USBio References
No references available