Technical Data

038997
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu Mo
Accession #
NP_003308.1
Gene ID
7080
Gene #
NKX2-1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NKX2-1, NT (NKX2-1, NKX2A, TITF1, TTF1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1)
NKX2-1, NKX2A, TITF1, TTF1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Indirect Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human NKX2-1. Species sequence homology: rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NKX2-1. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kim, J.H., et al. Acta Cytol. 54(3):277-282(2010) Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010) Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010) Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010) : Cantara, S., et al. Thyroid Res 3 (1), 4 (2010) :
USBio References
No references available
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