Technical Data

039127
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Gene ID
2908
Gene #
NR3C1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NR3C1, CT (Nuclear receptor subfamily 3 group C member 1, Glucocorticoid receptor, GR,GRL, NR3C1)
Nuclear receptor subfamily 3 group C member 1, Glucocorticoid receptor, GR,GRL, NR3C1

NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 712-742 from the C-terminal region of human NR3C1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010) |Szilagyi, K., et al. Neuro Endocrinol. Lett. 30(5):629-636(2009)|Tian, S., et al. Biochem. J. 367 (PT 3), 907-911 (2002) |Itoh, M., et al. Mol. Endocrinol. 16(10):2382-2392(2002)|Wang, Z., et al. J. Biol. Chem. 277(29):26573-26580(2002)
USBio References
No references available
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