039145
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E FC IHCCrossreactivity
HuGene ID
3084Gene #
NRG1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-NRG1, ID (NRG1, GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, membrane-bound isoform, Neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor)
NRG1, GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, membrane-bound isoform, Neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
Applications
Suitable for use in Immunohistochemistry, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (FFPE): 1:50-1:100 Flow Cytometry (Indirect): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 198-229 in the central region of human NRG1
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human NRG1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Stove, C., et al., J. Invest. Dermatol. 121(4):802-812 (2003). 2. Adelaide, J., et al., Genes Chromosomes Cancer 37(4):333-345 (2003). 3. Ritch, P.A., et al., J. Biol. Chem. 278(23):20971-20978 (2003). 4. Vermeer, P.D., et al., Nature 422(6929):322-326 (2003). 5. Atlas, E., et al., Mol. Cancer Res. 1(3):165-175 (2003).USBio References
No references available