Technical Data

039221
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_009103.2
Gene ID
10762
Gene #
NUP50
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NUP50, ID (NUP50, NPAP60L, Nuclear pore complex protein Nup50, 50kD nucleoporin, Nuclear pore-associated protein 60kD-like, Nucleoporin Nup50)
NUP50, NPAP60L, Nuclear pore complex protein Nup50, 50kD nucleoporin, Nuclear pore-associated protein 60kD-like, Nucleoporin Nup50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human NUP50.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
USBio References
No references available
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