Technical Data

039254
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_002692.2
Gene ID
5460
Gene #
POU5F1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-OCT3/4 (C279) (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3)
POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Abu-Remaileh, M., et al. EMBO J. 29(19):3236-3248(2010)|Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)|Schultz, S.S., et al. Mol. Cell. Biol. 30(18):4521-4534(2010)|Kim, S., et al. Br. J. Cancer 102(2):436-446(2010)|Wang, X., et al. Stem Cells 27(6):1265-1275(2009)
USBio References
No references available
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