Technical Data

039581-APC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu Mo
Accession #
NP_002553.2
Gene ID
5027
Gene #
P2RX7
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rabbit Anti-P2RX7, CT (P2RX7, P2X purinoceptor 7, ATP receptor, P2Z receptor, Purinergic receptor) (APC)
P2RX7, P2X purinoceptor 7, ATP receptor, P2Z receptor, Purinergic receptor

P2RX7 belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression.

Applications
Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 563-592 in the C-terminal region of human P2RX7
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human P2RX7. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kim, M., et al. EMBO J. 20(22):6347-6358(2001). 2. Gartland, A., et al. J. Bone Miner. Res. 16(5):846-856(2001). 3. Gu, B.J., et al. J. Biol. Chem. 276(14):11135-1114(2001). 4. Buell, G.N., et al. Recept. Channels 5(6):347-354(1998). 5. Rassendren, F., et al. J. Biol. Chem. 272(9):5482-5486(1997)
USBio References
No references available
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