Technical Data

039595
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Accession #
NP_001153014.1
Gene ID
5462
Gene #
POU5F1B
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-P5F1B, CT (POU5F1B, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, Putative POU domain, class 5, transcription factor 1B, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like)

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 1:1,000 Western Blot: 1:100-1:500 Indirect Flow Cytometry: 1:10-1:50 Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within aa214-242 from the C-terminal region of human P5F1B. Species sequence homology: bovine, porcine and mouse
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human P5F1B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kastler, S., et al. Prostate 70(6):666-674(2010)|White, K.L., et al. Twin Res Hum Genet 13(1):43-56(2010)|Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)|Gudmundsson, J., et al. Nat. Genet. 41(10):1122-1126(2009)|Panagopoulos, I., et al. Oncol. Rep. 20(5):1029-1033(2008)
USBio References
No references available
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